SFN Neuroblogging: The paralyzing effects of CO2

Sci is back from SFN, but she is by no means done with the neuroblogging! Unfortunately, due to a crazy schedule and spotty wireless, Sci was not able to get as much neuroblogging in as she wanted. So she’s going to continue for a few more days, with some of the coolest things she saw at this year’s conference.
For this post, we’re going to basic principles, made extra cool by two things: crayfish and videos!
Univ. Kentucky, Lexington, KY
Synaptic mechanisms underlying carbon dioxide’s induced paralysis

So, you might ask, why do an experiment in crawdads? Well, what the authors were studying here was the effects of carbon dioxide on paralysis. And though vertebrates (like us), and invertebrates (like crayfish) are very different, the effect of carbon dioxide is universal. If you increase the carbon dioxide the animal is exposed to, they will become paralyzed and pass out. The question is, why and how does this happen?
The basic mechanism is already known: when there’s high carbon dioxide present in the body, it will combine with water into two other chemicals, bicarbonate and protons. Bicarbonate is basic and can increase the body’s pH, but protons decrease pH. And if pH decreases far enough, the animal in question will pass out.
But the question is, how is this happening? What receptors and areas of the central nervous system are producing the effect? To examine this, the scientists looked in crayfish, and looked at various neurotransmitters, in particular, the neurotransmitter glutamate. They found that the presence of carbon dioxide in crayfish inhibits glutamate responses in the central nervous system, blocking chemical neurotransmission, but NOT blocking the electrical impulses going down the neurons. They monitored all of this by monitoring glutamate transmission, and also monitoring the heart rate and ventilation of the crayfish. This allowed the authors to look at whether or not the crayfish had passed out or was paralyzed by carbon dioxide, basic on the responses from the crayfish when you lightly tap on its tail. They could correlate these measures with the recordings they were also doing from neurons, to see how changes in neurotransmission correlated with the behavioral responses of the crayfish.
You might think, this is really pretty basic, and it is. But it’s also potentially very important, for humans and animals under conditions of high carbon dioxide, and could allow us to develop therapies to help under these conditions. And further understanding of this mechanism could also enable us to investigate how animals might differ when they are adapted to conditions of low pH.
And the coolest thing? They did this whole study in crayfish, with wires. And they made a VIDEO! Check it out as Sonya Bierbower shows you how to monitor heart rate and ventilation in crayfish, in a complete methods paper presented online! It’s a super cool new way to do things, and I highly recommend you check it out!

4 Responses

  1. Cool video, but is it too much to ask to get some frickin’ crayfish with some frickin’ laser beams on their rostra?

  2. Arvind: Heh, crayfish we don’t have. We DO, however, have virus-transferred receptors which can be injected into the brain, and which are activated by light of specific wavelengths, like lasers. So we do have MICE with laser beams attached to their heads. Or at least close to their heads.
    But are they…ill-tempered?

  3. Sci, it totally makes my day to see you stopped by Sonya and Dr. Cooper’s presentation. 😀

  4. Inside their heads? U R Doin’ it RONG!! If you get them trippy with a frickin’ laser show inside their heads, they’ll just go around with a silly grin on their faces singing kumbaya, hugging bonsai trees and bonking each other in largescale free love orgies!!

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